Analisis Bioautografi Kromatografi Lapis Tipis dan Aktivitas Antibakteri Ekstrak Etanol Bawang Tiwai (Eleutherine bulbosa (Mill.) Urb.) terhadap Methicillin-resistant Staphylococcus aureus (MRSA)

Authors

  • Swandari Paramita Laboratorium Kedokteran Komunitas, Fakultas Kedokteran, Universitas Mulawarman Author
  • Yadi Yasir Laboratorium Mikrobiologi, Fakultas Kedokteran, Universitas Mulawarman Author
  • Ibnu Sina Program Studi Pendidikan Dokter, Fakultas Kedokteran, Universitas Mulawarman Author

Keywords:

Eleutherine bulbosa, antimicrobial, MRSA, bioautography, thin layer chromatography

Abstract

Infeksi Methicillin-resistant Staphylococcus aureus (MRSA) merupakan masalah kesehatan utama di Indonesia, hal ini terutama karena resistensinya terhadap antibiotika. Berdasarkan hal tersebut maka upaya pencarian komponen antibakteri berbahan dasar alam saat ini menjadi fokus perhatian. Bawang tiwai (Eleutherine bulbosa (Mill.) Urb.) adalah salah satu yang memiliki potensi sebagai antibakteri berbahan dasar alam asli Indonesia. Bawang tiwai secara empiris sudah digunakan masyarakat lokal di Kalimantan Timur untuk mengobati berbagai penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol MRSA serta mengetahui jenis metabolit sekunder dengan aktivitas antibakteri yang diuji dengan metode bioautografi KLT. Penelitian dilakukan di Laboratorium Riset, Fakultas Kedokteran, Universitas Mulawarman, Samarinda. Hasil KLT menunjukkan bahwa ekstrak etanol E. bulbosa memiliki golongan senyawa yang mengandung gula, alkaloid, terpen-steroid dan flavonoid. Hasil bioautografi KLT menunjukkan bahwa ekstrak etanol E. bulbosa dapat menghambat pertumbuhan MRSA. Hasil KHM menunjukkan bahwa ekstrak etanol E. bulbosa memiliki potensi aktivitas antibakteri yang baik terhadap MRSA dengan angka KHM 1 mg/mL. Kesimpulan penelitian ini adalah seluruh hasil diatas mendukung potensi bawang dayak sebagai alternatif komponen antibakteri berbahan dasar alam terhadap MRSA.

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Published

2024-11-14

Issue

Vol. 1 No. 9 (2018): J. Sains Kes.

Section

Articles

How to Cite

[1]
S. Paramita, Y. Yasir, and I. Sina, “Analisis Bioautografi Kromatografi Lapis Tipis dan Aktivitas Antibakteri Ekstrak Etanol Bawang Tiwai (Eleutherine bulbosa (Mill.) Urb.) terhadap Methicillin-resistant Staphylococcus aureus (MRSA)”, J. Sains. Kes, vol. 1, no. 9, pp. 470–478, Nov. 2024, Accessed: Nov. 23, 2025. [Online]. Available: http://jsk.ff.unmul.ac.id/index.php/JSK/article/view/63

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