Potensi Penghambatan Enzim Tirosinase dengan Ekstrak Etanol Daun Kopasanda (Chromolaena Odorata L.)

Potential inhibition of Tyrosinase Enzyme by Ethanol Extracts Kopasanda Leaf (Chromolaena Odorata L.)

Authors

  • Astuti Amin Sekolah Tinggi Ilmu Farmasi Makassar, Makassar, Indonesia Author
  • Besse Hardianti Sekolah Tinggi Ilmu Farmasi Makassar, Makassar, Indonesia Author
  • Subehan Lallo Fakultas Farmasi, Universitas Hasanuddin, Makassar, Indonesia Author
  • Wahyu Hendrari Sekolah Tinggi Ilmu Farmasi Makassar, Makassar, Indonesia Author

DOI:

https://doi.org/10.30872/j.sains.kes.v6i1.280

Keywords:

Chromolaena odorata L, enzim tirosinase, Gulma

Abstract

Kebutuhan akan perawatan kulit semakin meningkat. Salah satu indikator kesehatan kulit adalah kecerahan kulit warna kulit. Enzim tirosinase dapat menggelapkan warna kulit karena aktivitasnya melawan biosintesis melanin. Warna kulit juga akan berubah ketika terpapar sinar UV, bahkan pada tingkat yang lebih parah dapat menyebabkan kanker. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas penghambatan enzim tirosinase dari ekstrak etanol daun kopasanda Chromolaena odorata L. Daun C. odorata merupakan tumbuhan gulma dari family Asteraceae yang mengandung beberapa senyawa seperti tannin, fenol, flavonoid, saponin, dan steroid. Pada penelitian ini dilakukan pengujian terhadap ekstrak etanol daun C. odorata dalam menghambat aktivitas enzim tirosinase berdasarkan persen penghambatannya. Penentuan aktivitas penghambatan enzim tirosinase menggunakan alat Spektrometer UV-Vis pada panjang gelombang 495 nm, dilakukan dengan menghitung nilai IC50 dengan asam kojic sebagai kontrol positif. Hasil penelitian menunjukkan bahwa nilai IC50 dari uji aktivitas penghambatan enzim tirosinase, asam kojic sebagai kontrol positif sebesar 27,472 mg L-1 (sangat kuat), sampel ekstrak etanol daun C.odorata  sebesar  81,546 mg L-1 (sangat kuat). Ekstrak etanol daun C. odorata memiliki efek penghambatan terhadap enzim tirosinase yang digunakan sebagai bahan sumber potensial senyawa antimelagenik alami.

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Published

2024-11-15

Issue

Vol. 6 No. 1 (2024): J. Sains Kes.

Section

Articles

How to Cite

Potensi Penghambatan Enzim Tirosinase dengan Ekstrak Etanol Daun Kopasanda (Chromolaena Odorata L.): Potential inhibition of Tyrosinase Enzyme by Ethanol Extracts Kopasanda Leaf (Chromolaena Odorata L.). (2024). Jurnal Sains Dan Kesehatan, 6(1), 132-141. https://doi.org/10.30872/j.sains.kes.v6i1.280