Detection of Flavonoid Compounds of Daruju Root Extract (Acanthus ilicifolius Linn) using Thin Layer Chromatography and UV-Vis Spectrophotometry

Authors

  • Aden Dhana Rizkita Sekolah Tinggi Ilmu Kesehatan Bogor Husada Author
  • Ilham Maulana Program Studi S-1 Farmasi, Sekolah Tinggi Ilmu Kesehatan Bogor Husada, Bogor, Indonesia Author
  • Sintia Ayu Dewi Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Semarang, Semarang, Indonesia Author

Keywords:

Daruja Root, Spektrofotometer UV-Vis, Flavonoid, TLC

Abstract

The isolation and detection of flavonoids in the extract of Daruju root has been carried out. extract obtained from maceration modification using 96% ethanol, ethanol extract The extraction result is concentrated with a rotary evaporator and then evaporated above water bath until a thick extract is obtained. The resulting extract was further separated using a separating funnel and diethyl ether and n-butanol as solvents. Then the thick extract was tested for plavonoid preliminaries by reacting the thick extract with H2SO4 and Mg the results obtained were a change in color to yellow, it proved that the extract was positive for plavonoid. After that, TLC was carried out to purify the extract which would later detect plavonoid using a UV-Vis spectrophotometer. TLC results show an Rf value of 0.6-0.8. UV-Vis spectrophotometer results at a wavelength of 200-800 nm give results in fraction A with a maximum wavelength at 291 nm which is suspected to have detected flavonoids.

References

Singh, D. And V. Aeri, 2013. Phytochemical and pharmacological potential of Acanthus ilicifolius. J. Pharm. Bioallied. Sci., 5: 17-20.

Dey, A., S.M. Raihan, H.M. Sariful Islam, M. Hamiduzzaman and A.S.M. Monjur-Al-Hossain, 2012. Phytochemical screening and the evaluation of the antioxidant, cytotoxic and antimicrobial properties of Acanthus ilicifolius (Family: Acanthaceae). Int. Res. J. Pharm., 3: 153-156.

Raut, S. And S. Khan, 2012. Phytochemical fingerprinting of Acanthus ilicifolius Linn. Adv. Plant Sci., 25: 749-753

Arumugam, S., Ramanathan, T., Kesavanarayanan, K.S., Adam, A. (2015). Traditional medicinal uses, chemical constituents and biological activities of a mangrove plant, acanthus ilicifolius linn.: a brief review.

Callemien, D., & Collin, S. (2008). Use of RP-HPLC-ESI(-)-MS/MS to differentiate various proanthocyanidin isomers in lager beer extracts. Journal of the American Society of Brewing Chemists, 66(2), 109–115

Feng, H.-L., Tian, L., Chai, W.-M., Chen, X.-X., Shi, Y., Gao, Y.-S., Yan, C.-L., & Chen, Q.-X. (2014). Isolation and purification of condensed tannins from flamboyant tree and their antioxidant and antityrosinase activities. Applied Biochemistry and Biotechnology, 173(1), 179–192.

AD Rizkita, E Cahyono, S Mursiti. 2017. Isolasi dan Uji Antibakteri Minyak Daun Sirih Hijau dan Merah Terhadap Streptococcus mutans. Indonesian Journal of Chemical Science. (3) 279-286

Dalimartha S. 2008, Atlas Tanaman Obat Indonesia. Jilid 6. Cetakan Revisi.Trubus Agriwidya. Jakarta

Ditjen POM. 1986, Departemen Kesehatan Indonesia, Sediaan Galenika, Jakarta

Kumar, K. T. M. S., Gorain, B., Roy, D. K., Zothanpuia, Samanta, S. K., Pal, M., Biswas, P., Roy, A., Adhikari, D., Karmakar, S., & Sen, T. (2008). Anti-inflammatory activity of Acanthus ilicifolius. Journal of Ethnopharmacology, 120(1), 7–12.

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Published

2023-02-28

Issue

Vol. 5 No. 1 (2023): J. Sains Kes.

Section

Articles

How to Cite

Detection of Flavonoid Compounds of Daruju Root Extract (Acanthus ilicifolius Linn) using Thin Layer Chromatography and UV-Vis Spectrophotometry. (2023). Jurnal Sains Dan Kesehatan, 5(1), 1-5. https://jsk.ff.unmul.ac.id/index.php/JSK/article/view/329