Determination of Total Flavonoid Content of Saputangan Leaves (Maniltoa grandiflora (A. Gray) Scheff) and Its Ability as Antioxidant

Authors

  • Jhon Patar Sinurat Institut Kesehatan Medistra Lubuk Pakam Author https://orcid.org/0000-0002-8142-5590
  • Reh Malem Br Karo Universitas Prima Indonesia Author
  • Rinaldo Berutu Institut Kesehatan Medistra Lubuk Pakam Author

Keywords:

total flavonoid content, screening of flavonoid, antioxidant, maceration, partition

Abstract

Determination of phenolic content using uv-visible and measured IC50 as antioxidant of saputangan leaves. Methods: Screening was used FeCl3, NH3, Alkali and Pb(CH3COO)2. The maceration process was carried out using ethanol as a solvent and soaked for more than 24 hours. The measurement of total flavonoid levels began with determining the maximum wavelength of quercetin, which was 442 nm. The linear regression equation for the resulting quercetin calibration curve was the equation y = 0.0155x + 0.0387 with a correlation value (R2) of 0.9943. The linear regression of antioxidant of saputangan leaves on percentage of inhibition is y = 0.6899x + 1.5616 with a correlation (R2) of 0.9895. The Maniltoa grandiflora (A. Gray) Scheff leaves contained flavonoid compounds which are proven based on the results of screening. Meanwhile, the weight of the extract after partitioning using ethyl acetate and n-hexane was 50 gr. Total flavonoid levels in Maniltoa grandiflora (A. Gray) Scheff leaves were 33.87 mg QE/g extract or 33.87%. IC50 value of 70.2108 ppm which was categorized as an antioxidant at the medium (medium) level. Conclusions: Total flavonoid content was 3.87 mg QE/g extract or 33.87% and categorized as antioxidant on medium level.

References

Maurice D. Awouafack, Pierre Tane and Hiroyuki Morita.2017. Isolation and Structure Characterization of Flavonoids. Intech. http://dx.doi.org/10.5772/67881

Hidayati N, Mansur M, Juhaeti T, 2013. Variasi Serapan Karbondioksida (CO2) Jenis-jenis Pohon di “Ecopark”, Cibinong dan Kaitannya dengan Potensi Mitigasi Gas Rumah Kaca. Pusat Penelitian Biologi LIPI. Cibinong.

Ika Buana Januarti, Hudan Taufiq and Sulistyaningsih. 2019. The Correlation of Total Flavonoid and Total Phenolic with Antioxidant Activity of Single Bulb Garlic (Allium Sativum) from Tawangmangu and Magetan. Jurnal Farmasi Sains Dan Komunitas, Vol. 16 No. 2. 96-103.

Sinurat JP, 2019. Antibakteri Senyawa Fenolik dari Daun Saputangan (Maniltoa grandiflora (A. Gray) Scheff). Jurnal Penelitian Farmasi dan Herbal OJS Deli Husada, 1: 17-21.

Elin Novia Sembiring, Berna Elya and Rani Sauriasari. 2018. Phytochemical Screening, Total Flavonoid and Total Phenolic Content and Antioxidant Activity of Di?erent Parts of Caesalpinia bonduc (L.) Roxb. Pharmacogn J ; 10(1): 123-127

Gusthinnadura Oshadie De Silva, Achala Theekshana Abeysundara and Malamige Minoli Weroshana Aponso. 2017. Extraction methods, qualitative and quantitative techniques for screening of phytochemicals from plants. American Journal of Essential Oils and Natural Products; 5(2): 29-32.

Farahziela Abu, Che Norma Mat Taib, Mohamad Aris Mohd Moklas and Sobri Mohd Akhir. 2017. Antioxidant Properties of Crude Extract, Partition Extract, and Fermented Medium of Dendrobium sabin Flower. Hindawi Evidence-Based Complementary and Alternative Medicine. Vol 2017; 1-8.

Sushant Aryal, Manoj Kumar Baniya, Krisha Danekhu, Puspa Kunwar, Roshani Gurung and Niranjan Koirala. 2019. Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants, 8, 96; doi:10.3390/plants8040096

Serban Moldoveanu and Victor David. 2015. Chapter 6: Solvent Extraction. Researchgate. https://www.researchgate.net/publication/282598341

Diah Astika Winahyu, Agustina Retnaningsih dan Marisa Aprillia. 2019. Penetapan Kadar Flavonoid pada Kulit Batang Kayu Raru (Cotylelobiummelanoxylonp) dengan Metode Spektrofotometri Uv-Vis. Jurnal Analis Farmasi. Volume 4, No. 1, Hal 29 – 36.

Ery al Ridho. 2013. Uji Aktivitas Antioksidan Ekstrak Metanol Buah Lakum (Cayratia Trifolia) Dengan Metode Dpph (2,2-Difenil-1-Pikrilhidrazil). Program Studi Farmasi Fakultas Kedokteran Universitas Tanjungpura Pontianak

Published

2024-11-14

How to Cite

Determination of Total Flavonoid Content of Saputangan Leaves (Maniltoa grandiflora (A. Gray) Scheff) and Its Ability as Antioxidant. (2024). Jurnal Sains Dan Kesehatan, 4(3), 275-279. https://jsk.ff.unmul.ac.id/index.php/JSK/article/view/553