Aktivitas Inhibitor Alfa-Amilase Beberapa Tumbuhan Obat Indonesia

Authors

  • Akhmad Jaizzur Rijai Fakultas Farmasi, Universitas Mulawarman, Samarinda-Kalimantan Timur Author
  • Asep Gana Suganda Sekolah Farmasi, Institut Teknologi Bandung Author
  • Elin Yulinah Iskandar Sekolah Farmasi, Institut Teknologi Bandung Author

Keywords:

alfa-amylase, traditional medicine plant, diabetes, IC50, aqueous extract

Abstract

Diabetes and its complication was major causes of death in most countries compared HIV/AIDS, tuberculosis and malaria. One approach in the treatment of diabetes is by slowing down the glucose absorption in the gastrointestinal tract, its can be achieved by inhibition of hydrolizing enzymes such as ?-amylase so the absorption of glucose becomes slow. This research aim was to determine the ?-amylase inhibitor activity from aqueous extract from some tradicional medicine plant such as daun insulin (Smallanthus sonchifolius (Poepp.) H.Rob), kembang bulan (Tithonia diversifolia (Hemsl.) Gray), bodhi leaf (Ficus religiosa L.), and Mahogany seeds (Swietenia mahagoni (L.) Jacq.). Extraction was done by reflux method using aquadest as a solvent. Extracts were obtained by freeze drying and tested for ?-amylase inhibitory activities. The IC50 value of daun insulin (EADI), kembang bulan leaf (EADKB), bodhi leaf (EADB) mahogany seeds (EABM) aqueous extract and acarbose as reference were 282,53; 473,85; 423,01; 220,91; and 3,42 ?g / mL, respectively.

References

[1]. Bhutkar, M. A., dan Bhise, S. B. (2012): In Vitro Assay of Alpha Amylase Inhibitory Activity of Some Indigenous Plants. Sadguru Publication, 10(1), 457-462.

[2]. Desmukh, T., Yadav, B., Badole, S., Bodhankar, S., dan Dhaneshwar, S., (2007): Antihyperglicaemic Activity of Alcoholic Extract of Ficus Religiosa Leaves in Alloxan Induced Diabetic Mice. Journal of Herbal Medicine and Toxicology. 1: 80-86.

[3]. Fransworth, N.R. (1966): Biological and Phytochemical Screening of Plants, Journal of Pharmaceutical Sciences, 55(3), 243-269.

[4]. Hajra, S., Mehta, A., Pandey, P., John, J., Mehta, P., dan Vyas, S.P. (2013): Free Radical Scavenging and ?-Amilase Inhibitory Activity of Swietenia mahagoni Seeds Oil. International Journal of Pharmacognosy and Phytochemical Research. 5(1): 51-56.

[5]. International Diabetes Federation. (2015): Diabetes Atlas, 7th Ed. International Diabetes Federation, 22-26.

[6]. Mentreddy S.R. (2007): Review Medicinal Plant Species with Potential Antidiabetic Properties. Journal of Science, Food and Agriculture, 87:743-750.

[7]. Miura, T, Nosaka, K., Ishii, H., dan Ishida, T. (2005): Antidiabetic Effect of Nitobegiku, The Herb Tithonia diversifolia, in KK-Ay Diabetic Mice. Biological and Pharmaceutical Bulletin. 28(11): 2152-2154.

[8]. Mun’im, A., Katrin, Azizahwati, Andriani, A., Mahmudah K.F., dan Mashita, M. (2013): Screening of ?-Glucosidase Inhibitory Activity of Some Indonesia Medicinal Plants. International Journal of Medicinal Aromatic Plants. 3: 144-150.

[9]. Moghadamtousi, S.Z., Goh, B.H., Chan, C.K., Shabab, T., dan Kadir, H.A. (2013): Biological Activites and Phytochemicals of Swietenia macrophylla King. Molecules. 18: 10465-10483.

[10]. Odhav, B., Kandasamy, T., Khumalo, N., dan Baijnath, H. (2010): Screening of African Traditional Vegetables for Their alpha-amylase Inhibitory Effect. Journal of Medicinal Plant Research 4(14), 1502-1507.

[11]. Shori, A.B., dan Baba, A.S. (2011): Antioxidant Activity and Inhibition of Key Enzym Linked to Type-2 Diabetes and Hypertension by Azadirachta indica-yogurt. Journal of Saudi Chemical Society, 17: 295-301.

[12]. Soraya Riyanti, Asep Gana suganda, Elin yulinah Iskandar (2016): Dipeptidyl Peptidase-IV Inhibitory Activity of Some Indonesian Medicinal Plants. Asian Journal of Pharmaceutical and Clinical Research Vol 9, Issue 2, 375-377.

[13]. Susana, B.G., Wilfredo, M.C., Marua, I.M., Alfredo, G., Cesar, A.C., dan Sara, S.S. (2010): Hypoglycemic Activity Of Leaf Organic Extracts From Smallanthus sonchifolius: Constituents Of The Most Active Fraction. Chemical-Biological Interaction, 185, 143-152

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Published

2024-11-14

Issue

Vol. 1 No. 10 (2018): J. Sains Kes.

Section

Articles

How to Cite

[1]
A. J. Rijai, A. G. Suganda, and E. Y. Iskandar, “Aktivitas Inhibitor Alfa-Amilase Beberapa Tumbuhan Obat Indonesia”, J. Sains. Kes, vol. 1, no. 10, pp. 517–524, Nov. 2024, Accessed: Oct. 30, 2025. [Online]. Available: https://jsk.ff.unmul.ac.id/index.php/JSK/article/view/122

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