Aktivitas Antibakteri Kokristal Sefiksim-Nikotinamida Menggunakan Agar Diffusion dan Broth Dilution Test

Authors

Abstract

Sefiksim telah berhasil dibuat dalam bentuk kokristal dengan nikotinamida (koformer). Kokristal SEF-NIK dibuat dengan perbandingan mol 1:1 menggunakan metode liquid-assisted grinding­. Kokristal dikarakterisasi menggunakan DSC, FTIR, SEM, dan PXRD. Hasil karakterisasi menunjukkan bahwa terbentuk kokristal antara sefiksim dan nikotinamida. Hasil uji kelarutan, laju disolusi, dan permeabilitas kokristal SEF-NIK menunjukkan peningkatan yang signifikan dibandingkan dengan sefiksim murni. Berdasarkan hasil penelitian tersebut, kokristal SEF-NIK dilanjutkan ke tahap uji farmakodinamik yakni uji aktivitas antibakteri secara in vitro. Penelitian ini bertujuan untuk mengetahui pengaruh dari teknik kokristalisasi terhadap aktvitas antibakteri sefiksim. Pengujian dilakukan menggunakan dua metode yakni Agar Diffusion Method (difusi agar) dan Broth Dilution Method (dilusi cair). Hasil penelitian menunjukkan bahwa kokristalisasi sefiksim dengan nikotinamida tidak menghambat efektivitasnya terhadap bakteri Escherichia coli, akan tetapi efektivitasnya lebih baik dibandingkan sefiksim murni. Pada metode difusi agar, zona hambat sefiksim terhadap bakteri Escherichia coli sebesar 6,2 mm sedangkan kokristal SEF-NIK lebih besar yakni 8,6 mm. Pada metode dilusi cair, hasil pengukuran OD bakteri Escherichia coli hari kelima pada sefiksim sebesar 1,330 sedangkan pada kokristal SEF-NIK sebesar 1,064.

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Published

2024-11-14

Issue

Vol. 5 No. 6 (2023): J. Sains Kes.

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Articles

How to Cite

Aktivitas Antibakteri Kokristal Sefiksim-Nikotinamida Menggunakan Agar Diffusion dan Broth Dilution Test. (2024). Jurnal Sains Dan Kesehatan, 5(6), 886-892. https://jsk.ff.unmul.ac.id/index.php/JSK/article/view/394