Karakterisasi Fungi Endofit Syzygium polyanthum (Wight) Walp. Berdasarkan Gen ITS Sebagai Penghasil Senyawa Antibakteri

Endophytic Fungi Characterization Syzygium polyanthum (Wight) Walp. Based on ITS Genes as Producers of Antibacterial Compounds

Penulis

  • Asti Vebriyanti Asjur Universitas Megarezky, Makassar Penulis

Kata Kunci:

Syzygium polyanthum, fungi endofit, antimikroba, analisis filogenetik, Penicillium sp. Colletotrichium

Abstrak

Penelitian ini diawali dengan menumbuhkan dari daun salam yang telah disterilkan menggunakan etanol 70% dan natrium hipoklorit. Skrining antibakteri dilakukan dengan menggunakan uji antagonis sebagai screening pertama melawan Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Salmonella thyposa. Penelitian ini menghasilkan 2 isolat fungi. Fungi endofit aktif difermentasi dan ekstraksi dengan etil asetat (EtOAc) dan metanol. Aktivitas antibakteri dari ekstrak EtOAc dan metanol diuji aktivitasnya menggunakan metode difusi disk. Satu isolat fungi endofit (XP1) terhadap lima dari bakteri yang diuji adalah S. aureus, P. Aeruginosa, E. Coli, S. thyposa, dan B.subtilis, memiliki daya hambat terbesar pada bakteri uji S. thyposa dengan diameter daya hambat sebesar 14,00 mm. Sedangkan pada isolat fungi endofit (XP2) terhadap lima dari bakteri yang diuji adalah S. aureus, P. Aeruginosa, E. Coli, S. thyposa, dan B.subtilis, memiliki daya hambat terbesar pada bakteri uji S. thyposa dengan diameter daya hambat sebesar 22,00 mm. Berdasarkan analisis filogenetik isolat XP 1 memiliki kemiripan dengan Penicillium sp. (homology 87%) sedangkan isolat XP2 memiliki kemiripan dengan Colletotrichium gloeosporioides (homology 98%).

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Unduhan

Diterbitkan

2024-11-14

Terbitan

Vol 3 No 3 (2021): J. Sains Kes.

Bagian

Articles

Cara Mengutip

Karakterisasi Fungi Endofit Syzygium polyanthum (Wight) Walp. Berdasarkan Gen ITS Sebagai Penghasil Senyawa Antibakteri: Endophytic Fungi Characterization Syzygium polyanthum (Wight) Walp. Based on ITS Genes as Producers of Antibacterial Compounds. (2024). Jurnal Sains Dan Kesehatan, 3(3), 404-409. https://jsk.ff.unmul.ac.id/index.php/JSK/article/view/223