Profil Aktivitas Enzim Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), dan Alanine Transaminase (ALT) Pada Tikus Hiperlipidemia yang Diberi Madu Fermentasi Bawang Tunggal

Authors

  • Devyana Dyah Wulandari Universitas Nahlatul Ulama Surabaya Author
  • Suprapto Ma'at Department of Medical Laboratory Technology, Faculty of Health Author
  • Lysa Veterini Department of Medicine, Faculty of Medicine Author
  • Devi Fitriana Sari Department of Medical Laboratory Technology, Faculty of Health Author
  • Devyani Diah Wulansari Department of Medicine, Faculty of Medicine Author

DOI:

https://doi.org/10.30872/jsk.v6i1.636

Keywords:

Hiperlipidemia, Madu fermentasi, ALP, AST, ALT, Diet tinggi lemak

Abstract

Madu terfermentasi dengan bawang putih telah diketahui memiliki kandungan senyawa fenolat yang tinggi. Penelitian ini bertujuan untuk mengetahui efek suplementasi madu fermentasi terhadap profil aktivitas enzim Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), dan Alanine Transaminase (ALT) pada Tikus Hiperlipidemia.  Penelitian ini menggunakan 24 ekor tikus jantan dengan berat badan 200-250 dibagi menjadi 6 kelompok yaitu kontrol negatif, kontrol positif, P1 yang diberi madu fermentasi dosis 0,2 mg/kg BB/hari, kelompok P2 yang diberi madu fermentasi dosis 0,5 mg/kg BB/hari, kelompok P3 yang diberi madu fermentasi dosis 1 mg/kg BB/hari dan kelompok P4 yang diberi simvastatin dosis 1.008 g/l. Kadar ALP, AST, dan ALT serum diukur dengan fotometer. Hasil penelitian menunjukkan nilai signifikansi ALP p=0,152, AST p=0,839, dan ALT p=0,025 dengan p<0,05. Dapat disimpulkan bahwa kadar ALP dan AST yang diberikan madu fermentasi tidak memberikan pengaruh yang signifikan, sedangkan kadar ALT yang diberikan madu fermentasi memberikan pengaruh yang signifikan.

References

[1] Nuralifah, N., Wahyuni, W., Parawansah, P., & Shintia, U. D. (2020). Uji Aktivitas Antihiperlipidemia Ekstrak Etanol Daun Notika (Arcboldiodendron calosericeum Kobuski) Terhadap Kadar Kolesterol Total Tikus (Rattus norvegicus) Jantan Galur Wistar. Journal Syifa Sciences and Clinical Research, 2(1), 1-10.

[2] Chairunnisa, N.H. (2015). Efectivity of Roselle Extract (Hibiscus sabdariffa L.) as Treatment For Hyperlipidemia, Jurnal Majority, 4(4)

[3] WHO (2019). Cardiovascular Diseases. World Health Organization https://www.who.int/health-topics/cardiovascular-diseases/

[4] Riset Kesehatan Dasar (Riskesdas) (2018). Badan Penelitian dan Pengembangan Kesehatan Kementerian RI tahun 2018. http://www.depkes.go.id/resources/download/infoterkini/materi_rakorpop_20 18/Hasil%20Riskesdas%202018.pdf

[5] Reza Ahmad, Rachmawati Banundari. 2017. Difference SGOT And SGPT Level Between Subject With And Without Diabetes Mellitus. Jurnal Kedokteran Diponegoro. Vol. 6, No. 2:158-166

[6] Lala V, Zubair M, Minter DA. Liver Function Tests. [Updated 2023 Jul 30]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK482489/

[7] Rahmawan, A. A., & Wijayanti, T. (2016). Grant Of Influence Of Pandan Leaves (Pandanus amaryllifolius Roxb.) Ethanol Extract To Rate Alp In White Rats Isoniazid And Rifampisin Induced. Jurnal Farmasi Indonesia, 13(1), 41–50.

[8] Becerril-Sánchez, A. L., Quintero-Salazar, B., Dublán-García, O., & Escalona-Buendía, H. B. (2021). Phenolic Compounds in Honey and Their Relationship with Antioxidant Activity, Botanical Origin, and Color. Antioxidants, 10(11), 1700. https://doi.org/10.3390/antiox10111700Brouwer, J. V., Wirjatmadi, R. B., & Adriani, M. (2018). Single Cloves Garlic Extract in Enzyme Lipoprotein Lipase Activity of White Rats with High Fat Diet. Jurnal Ilmiah Kedokteran Wijaya Kusuma, 7(2), 126–132.

[9] Wulansari,D. D., Wulandari, D. D., & Krisdayanti, A., “Comparative Study Of In-Vitro Toxicity Of Pure Honey And Fermented Honey Using The BSLT (Brine Shrimp Lethality Test) Method” Register: Medical Technology and Public Health Journal, Vol. 6, No. 2, pp. 148-156, 2022

[10] Wisnu, W., Alwi, I., Nafrialdi, N., Pemayun, T. G. D., Pantoro, N. I., Wijaya, C. N., Tahapary, D. L., Tarigan, T. J. E., & Subekti, I. (2024). The Effects of Anti-thyroid Drugs on Lipoproteins and Insulin Resistance in Graves' Disease: A Randomized Clinical Trial. Journal of lipid and atherosclerosis, 13(3), 358–370. https://doi.org/10.12997/jla.2024.13.3.358

[11] Shehab, N. G., Abu-Gharbieh, E., & Bayoumi, F. A. (2015). Impact of phenolic composition on hepatoprotective and antioxidant effects of four desert medicinal plants. BMC complementary and alternative medicine, 15, 401. https://doi.org/10.1186/s12906-015-0919-6

[12] Umamit, F. A., Haruna, N., & Darmawansyih, D. (2021). Pengaruh Pemberian Madu terhadap Kadar Kolesterol Mencit (Mus musculus) dengan Salmonella Typhii. Molucca Medica, 14(April), 70–75. https://doi.org/10.30598/molmed.2021.v14.i1.70

[13] Brouwer, J.V., Wirjatmadi, R.B., Adriani, M. (2018). Ekstrak Bawang Putih Siung Tunggal terhadap Aktivitas Enzim Lipoprotein Lipase pada Tikus dengan Diet Tinggi Lemak. Jurnal Ilmiah Kedokteran Wijaya Kusuma 7(2) : 126-132

[14] Talreja O, Kerndt CC, Cassagnol M. Simvastatin. [Diperbarui 5 Juni 2023]. Dalam: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Tersedia dari: https://www.ncbi.nlm.nih.gov/books/NBK532919/

[15] Mahmudul Hasan KM, Tamanna N, Anwarul Haque M. Biochemical and histopathological profiling of Wistar rat treated with Brassica napus as a supplementary feed. Food Science and Human Wellness, 2018, 7(1): 77

[16] Prahastuti, S., Ladi, J. E., Dewi, K., Albertina, F., & Imam, M. K. (2020). The effect of bee pollen on SGOT, SGPT levels and liver histopathological images of male rats wistar induced by high fat diet. Journal of Medicine and Health, 2(5).

[17] Wulandari, D. D. (2017). Analisa Kualitas Madu (Keasaman, Kadar Air, dan Kadar Gula Pereduksi) Berdasarkan Perbedaan Suhu Penyimpanan. Jurnal Kimia Riset, 2(1), 16. https://doi.org/10.20473/jkr.v2i1.3768

[18] Ali, A. M., & Kunugi, H. (2019). Bee honey protects astrocytes against oxidative stress: A preliminary in vitro investigation. Neuropsychopharmacology reports, 39(4), 312–314. https://doi.org/10.1002/npr2.12079

[19] Valgimigli L. (2023). Lipid Peroxidation and Antioxidant Protection. Biomolecules, 13(9), 1291. https://doi.org/10.3390/biom13091291

[20] Yuliana, A. R., & Ardiaria, M. (2016). Effect Of Red Pitaaya (Hylocereus polyrhiziu) On Triglyserides Of Male White Rats (Rattus norvegicus) That Given Hight Fat Diet. Journal of Nutrition College, 5(4), 428–437. https://doi.org/10.14710/jnc.v5i4.16454

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Published

2025-05-31

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Vol. 6 No. 1 (2025)

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How to Cite

[1]
D. Dyah Wulandari, S. Ma'at, L. Veterini, D. F. Sari, and D. D. Wulansari, “Profil Aktivitas Enzim Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), dan Alanine Transaminase (ALT) Pada Tikus Hiperlipidemia yang Diberi Madu Fermentasi Bawang Tunggal”, J. Sains. Kes, vol. 6, no. 1, pp. 51–58, May 2025, doi: 10.30872/jsk.v6i1.636.

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