Uji Aktivitas Antijamur Ekstrak Etil Asetat Buah Terong Ungu (Solanum melongena L.) terhadap Jamur Candida tropicalis

Antifungal Activity of Ethyl Acetate Extract of Purple Eggplant (Solanum melongena L.) Fruit Against Candida tropicalis

Penulis

  • Fitriani Nurbaeti Program Studi Pendidikan Dokter, Fakultas Kedokteran, Universitas Jenderal Soedirman, Indonesia Penulis
  • Setiawati Setiawati Departemen Farmakologi, Fakultas Kedokteran, Universitas Jenderal Soedirman, Indonesia Penulis
  • Nia Krisniawati Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Jenderal Soedirman, Indonesia Penulis
  • Eman Sutrisna Departemen Farmakologi, Fakultas Kedokteran, Universitas Jenderal Soedirman, Indonesia Penulis https://orcid.org/0000-0001-6169-9650

Kata Kunci:

Candida tropicalis, terong ungu, antijamur

Abstrak

Candida tropicalis (C. tropicalis) telah ditetapkan sebagai emergency pathogenic. Buah terong ungu mengandung senyawa metabolit sekunder yang berkhasiat sebagai anti mikroba. Penelitian ini bertujuan untuk mengetahui besarnya Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) ekstrak etil asetat buah terong ungu terhadap pertumbuhan jamur C. tropicalis. Desain penelitian ini yaitu true experimental secara in vitro dengan perlakuan terdiri atas kontrol negatif (tanpa pemberian jamur), kontrol media RPMI, Ekstrak 40 mg/mL, 20 mg/mL, 10 mg/mL, 5 mg/mL, dan kontrol pelarut DMSO 10%. Penentuan KHM dilakukan melalui pengamatan langsung menggunakan metode microbroth dilution dalam mikroplate 96 well. Penentuan KBM menggunakan teknik spread plate yang dihitung dengan rumus Total Plate Count (TPC). Ekstrak etil asetat buah terong ungu memiliki KHM dan KBM yang sama yaitu pada konsentrasi 20 mg/mL. Konsentrasi ekstrak 20 mg/mL sudah mampu memberikan hambatan sebesar 100% terhadap pertumbuhan jamur C. tropicalis. Daya hambat pertumbuhan jamur semakin besar seiring dengan peningkatan dosis ekstrak. Ekstrak etil asetat buah terong ungu memiliki potensi untuk dikembangkan sebagai antijamur terhadap jamur C. tropicalis.

Referensi

Dewayanti W. Efektivitas Kunyit (Curcuma Longa Linn) sebagai Antijamur. Jurnal Medika Hutama. 2022;03:2019–24.

Djarot prasetyorini, Utami NF, Novitasari N, Fitriyani W. Potensi Ekstrak Refluks Kulit Batang Kayu Manis (Cinnamomum brmannii) sebagai Antijamur Candida albicans dan Candida tropicalis. Jurnal Ilmiah Farmasi. 2021;11:164–78.

Singh DK, Tóth R, Gácser A. Mechanisms of Pathogenic Candida Species to Evade the Host Complement Attack. Front Cell Infect Microbiol. Frontiers Media S.A.; 2020.

Wulandari A, Hapsari R, Sari D, Puspitasari I, Tugasworo Pramukarso D. Antifungal susceptibility profile of Candida spp. causing candidemia in an Indonesian tertiary hospital [Internet]. Indonesian Society for Clinical Microbiology | JCMID. 2021. Available from: https://jcmid.id/index.php/JCMID

Chatrath A, Gangwar R, Kumari P, Prasad R. In vitro anti-biofilm activities of citral and thymol against Candida tropicalis. Journal of Fungi. 2019;5.

Febriza A, Faradiana S, Yusbar Y, Kasim VN. Antifungal Effects of Solanum Melongena L Peel Extract Against Candida Albicans: In Vitro Study. 2021. p. 1–7.

Handayani MS, Setiawati S, Krisnawati N, Sutrisna eman. Uji Aktivitas Antibakteri Ekstrak Etil Asetat Terong Ungu (Solanum melongena L) terhadap bakteri escherichia coli. Medical and Helath Journal. 2023;2:102–9.

Zein ANS, Setiawati S, Krsiniawati N, Sutrisna E. Uji Aktivitas Antibakteri Ekstrak Etil Asetat Terong Ungu (Solanum melongena L.) terhadap Bakteri Staphylococcus epidermidis ATCC 12228. Jurnal Sains dan Kesehatan. 2023;5:157–63.

Putri RS, Setiawati S, Setyono J, Sutrisna eman, Mardhihusodo HR. Uji Aktivitas Antibakteri ekstrak etil Asetat Terong Ungu (Solanum melongena L) terhadap Bakteri Satphylococcus aureus. Jurnal Sains dan Kesehatan. 2023;5:205–11.

Setiawati S, Nuryastuti T, Ngatidjan N, Mustofa M, Jumina J, Fitriastuti D. In vitro antifungal activity of (1)-N-2-methoxybenzyl-1,10-phenanthrolinium bromide against Candida albicans and its effects on membrane integrity. Mycobiology. 2017;45.

Montes K, Ortiz B, Galindo C, Figueroa I, Braham S, Fontecha G. Identification of candida species from clinical samples in a honduran tertiary hospital. Pathogens. 2019;8.

El-Shabrawy AAEM, Abd El-Sattar MAEA, Emara AS, El-Ashry MAER, Fahim NAE. Screening for anti-fungal resistance in Candida species using chromogenic agar dilution. Microbes and Infectious Diseases. 2024;5:807–17.

Edziri H, Ammar S, Souad L, Mahjoub MA, Mastouri M, Aouni M, et al. In vitro evaluation of antimicrobial and antioxidant activities of some Tunisian vegetables. South African Journal of Botany. 2012;78:252–6.

Sumitra Devi L, Maheshwari M. Speciation of Candida Species Isolated From Clinical Specimens by Using Chrom Agar and Conventional Methods. International Journal of Scientific and Research Publications [Internet]. 2014;4. Available from: www.ijsrp.org

Hazen KC. Fungicidal versus fungistatic activity of terbinafine and itraconazole: An in vitro comparison. 1998.

Edziri H, Ammar S, Souad L, Mahjoub MA, Mastouri M, Aouni M, et al. In vitro evaluation of antimicrobial and antioxidant activities of some Tunisian vegetables. South African Journal of Botany. 2012;78:252–6.

Koponen JM, Happonen AM, Mattila PH, Törrönen AR. Contents of anthocyanins and ellagitannins in selected foods consumed in Finland. J Agric Food Chem. 2007;55:1612–9.

Whitaker BD, Stommel JR. Distribution of hydroxycinnamic acid conjugates in fruit of commercial eggplant (Solanum melongena l.) cultivars. J Agric Food Chem. 2003;51:3448–54.

Jawad Kadhim N, Sareea Al-Rekaby L, Abdul Redha A, Chappell J. Chemical Composition and Antioxidant Capacity of Eggplant Parts during Vegetative and Flowering Stage. J Phys Conf Ser. Institute of Physics Publishing; 2019.

Aboody MS Al, Mickymaray S. Anti-fungal efficacy and mechanisms of flavonoids. Antibiotics. MDPI AG; 2020.

Chowa?ski S, Adamski Z, Marciniak P, Rosi?ski G, Büyükgüzel E, Büyükgüzel K, et al. A review of bioinsecticidal activity of Solanaceae alkaloids. Toxins (Basel). MDPI AG; 2016.

Dhamgaye S, Devaux F, Vandeputte P, Khandelwal NK, Sanglard D, Mukhopadhyay G, et al. Molecular mechanisms of action of herbal antifungal alkaloid berberine, in Candida Albicans. PLoS One. 2014;9.

Salamatullah AM, Alkaltham MS, Hayat K, Ahmed MA, Arzoo S, Husain FM, et al. Bioactive and antimicrobial properties of eggplant (Solanum melongena l.) under microwave cooking. Sustainability (Switzerland). 2021;13:1–12.

Atanasov AG, Zotchev SB, Dirsch VM, Orhan IE, Banach M, Rollinger JM, et al. Natural products in drug discovery: advances and opportunities. Nat Rev Drug Discov. Nature Research; 2021. p. 200–16.

Unduhan

Diterbitkan

2024-11-14

Terbitan

Vol 6 No 3 (2024): J. Sains Kes.

Bagian

Articles

Cara Mengutip

Uji Aktivitas Antijamur Ekstrak Etil Asetat Buah Terong Ungu (Solanum melongena L.) terhadap Jamur Candida tropicalis: Antifungal Activity of Ethyl Acetate Extract of Purple Eggplant (Solanum melongena L.) Fruit Against Candida tropicalis. (2024). Jurnal Sains Dan Kesehatan, 6(3), 405-412. https://jsk.ff.unmul.ac.id/index.php/JSK/article/view/34