Aktivitas Antiinflamasi In Vitro dan In Vivo Ekstrak Etanol Daun Mangga Arumanis (Mangifera indica L.)

In Vitro and In Vivo Anti-Inflamatory Activities of Ethanol Extract Mangifera indica L. Leaves

Authors

  • Sholihatil Hidayati Program Studi Farmasi, Fakultas Ilmu Kesehatan, Universitas dr. Soebandi Author
  • Firda Oktavianti Program Studi Farmasi, Fakultas Ilmu Kesehatan, Universitas dr. Soebandi Author
  • Dhina Ayu Susanti Program Studi Farmasi, Fakultas Ilmu Kesehatan, Universitas dr. Soebandi Author
  • Qurrotul Aini Program Studi Farmasi, Fakultas Ilmu Kesehatan, Universitas dr. Soebandi Author

Keywords:

Mangifera Indica L., antiinflamation, in vitro, in vivo, leaves extract

Abstract

Inflammation is a normal process designed to protect oneself and promote healing of body tissues. The mechanism of action of flavonoids as antiinflammatory is through the inhibition of arachidonic acid and the secretion of lysosomal enzymes. The ethanol extract of Mangifera indica L. leaves (EEML) contains flavonoids and is widely used as a medicine empirically. This study was conducted to evaluate the antiinflammatory effect of EEML in vitro and in vivo. In vitro study of the antiinflammatory activity of EEML based on its effect on the stability of blood membranes using UV-Vis spectrophotometry method. While the in vivo activity was tested using the carrageenan induction method on white rats of the Wistar strain. In vitro activity was expressed as EC50 and in vivo activity was expressed as % edema reduction. The results showed that ethanol extract was able to increase membrane stability with an EC50 value of 8.56 ?g/ml better than positive control aspirin with EC50 21.57 ?g/ml. In vivo test results showed that the administration of EEML doses of 200 mg/kg.bw, 400 mg/kg.bw, and 800 mg/kg.bw was not significantly different from the positive control in reducing edema. EEML has shown antiinflammatory effects in vitro and in vivo, so it has the potential to be developed as an antiinflammatory agent.

References

D. E. Becker, “Basic and clinical pharmacology of Glucocorticosteroids,” Anesth. Prog., vol. 60, no. 1, pp. 25–32, 2013, doi: 10.2344/0003-3006-60.1.25.

D. Furman et al., “Chronic inflammation in the etiology of disease across the life span,” Nat. Med., vol. 25, no. 12, pp. 1822–1832, 2019, doi: 10.1038/s41591-019-0675-0.

S. V. Stankov, “Definition of Inflammation, Causes of Inflammation and Possible Anti-inflammatory Strategies,” Open Inflamm. J., vol. 5, no. 1, pp. 1–9, 2012, doi: 10.2174/1875041901205010001.

L. A. Abdulkhaleq, M. A. Assi, R. Abdullah, M. Zamri-Saad, Y. H. Taufiq-Yap, and M. N. M. Hezmee, “The crucial roles of inflammatory mediators in inflammation: A review,” Vet. World, vol. 11, no. 5, pp. 627–635, 2018, doi: 10.14202/vetworld.2018.627-635.

O. O. Oguntibeju, “Medicinal plants with anti-inflammatory activities from selected countries and regions of africa,” J. Inflamm. Res., vol. 11, pp. 307–317, 2018, doi: 10.2147/JIR.S167789.

Y. Umi, H. Siti, O. Winda, and C. Ratu, “Aktivitas Antiinflamasi Ekstrak Etanol Buah dan Asam Jawa (Tamarindus indica) Serta Kombinasinya pada Tikus Jantan Galur Wistar,” pp. 83–88, 2015.

K. A. Shah, M. B. Patel, R. J. Patel, and P. K. Parmar, “Mangifera indica (mango),” Pharmacogn. Rev., vol. 4, no. 7, p. 42, 2010.

S. Hidayati, S. Shinta Mayasari, L. Setyaningrum, A. D. Wardani, and Q. Aini, “In vitro antidiabetic activity of Peperomia pellucida extract and fraction by alpha-amylase inhibition pathway,” Pharmaciana, vol. 12, no. 2, p. 156, 2022, doi: 10.12928/pharmaciana.v12i2.21874.

K. Kosala, M. A. Widodo, S. Santoso, and S. Karyono, “In vitro and in vivo anti-inflammatory activities of Coptosapelta flavescens Korth Root’s methanol extract,” J. Appl. Pharm. Sci., vol. 8, no. 9, pp. 42–48, 2018, doi: 10.7324/JAPS.2018.8907.

T. W. Senduk, L. A. D. Y. Montolalu, and V. Dotulong, “The rendement of boiled water extract of mature leaves of mangrove Sonneratia alba,” J. Perikan. DAN Kelaut. Trop., vol. 11, no. 1, pp. 9–15, 2020.

E. Ukieyanna, “Aktivitas antioksidan, kadar fenolik, dan flavonoid total tumbuhan suruhan (Peperomia pellucida L. Kunth),” Skripsi. Fak. Mat. dan Ilmu Pengetah. Alam. Inst. Pertan. Bogor, 2012.

A. V. A. David, R. Arulmoli, and S. Parasuraman, “Overviews of biological importance of quercetin: A bioactive flavonoid,” Pharmacogn. Rev., vol. 10, no. 20, p. 84, 2016.

A. Vafadar et al., “Quercetin and cancer: New insights into its therapeutic effects on ovarian cancer cells,” Cell Biosci., vol. 10, no. 1, pp. 1–17, 2020, doi: 10.1186/s13578-020-00397-0.

T. yang Wang, Q. Li, and K. shun Bi, “Bioactive flavonoids in medicinal plants: Structure, activity and biological fate,” Asian Journal of Pharmaceutical Sciences, vol. 13, no. 1. Shenyang Pharmaceutical University, pp. 12–23, Jan. 01, 2017, doi: 10.1016/j.ajps.2017.08.004.

E. Umapathy et al., “An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation,” J. Med. Plants Res., vol. 4, no. 9, pp. 789–795, 2010, doi: 10.5897/JMPR10.056.

R. Ginwala, R. Bhavsar, D. G. I. Chigbu, P. Jain, and Z. K. Khan, “Potential role of flavonoids in treating chronic inflammatory diseases with a special focus on the anti-inflammatory activity of apigenin,” Antioxidants, vol. 8, no. 2, pp. 1–28, 2019, doi: 10.3390/antiox8020035.

M. Audina and K. Khaerati, “Efektivitas Antiinflamasi Ekstrak Etanol Daun Sumambu (Hyptis capitata Jacq.) Pada Tikus Jantan (Rattus norvegicus L.),” Bocelebes, vol. 12, no. 2, pp. 17–23, 2018.

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Published

2022-10-31

Issue

Vol. 4 No. 5 (2022): J. Sains Kes.

Section

Articles

How to Cite

[1]
S. Hidayati, F. Oktavianti, D. A. Susanti, and Q. Aini, “Aktivitas Antiinflamasi In Vitro dan In Vivo Ekstrak Etanol Daun Mangga Arumanis (Mangifera indica L.): In Vitro and In Vivo Anti-Inflamatory Activities of Ethanol Extract Mangifera indica L. Leaves”, J. Sains. Kes, vol. 4, no. 5, pp. 488–494, Oct. 2022, Accessed: Jul. 14, 2026. [Online]. Available: https://jsk.ff.unmul.ac.id/index.php/JSK/article/view/546

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