Isolation and Toxicity Test of Chitosan from Green Mussels (Perna viridis L.) With Brine Shrimp Lethality Test (BSLT) Method

Study Program of Pharmacy, Faculty of Pharmacy, Universitas Muslim Nusantara Al Washliyah, Medan, Indonesia

Penulis

  • Ridwanto Study Program of Pharmacy, Faculty of Pharmacy, Universitas Muslim Nusantara Al Washliyah, Medan, Indonesia Penulis
  • Audry Pratiwi Study Program of Pharmacy, Faculty of Pharmacy, Universitas Muslim Nusantara Al Washliyah, Medan, Indonesia Penulis
  • Zulmai Rani Study Program of Pharmacy, Faculty of Pharmacy, Universitas Muslim Nusantara Al Washliyah, Medan, Indonesia Penulis https://orcid.org/0000-0003-4547-9054

Kata Kunci:

BSLT, Chitosan, Shellfish, Toxicity Test

Abstrak

Green Mussels (Perna viridis L.) is one of Indonesia's fishery resources. The purpose of this study was to determine the toxicity level of chitosan from green clam shells (Perna viridis L.) by looking at the LC50 value tested on the BSLT method. This study included isolation of chitin and chitosan: deproteination, demineralization, depigmentation and deacetylation of chitin into chitosan, chitosan characterization, FTIR, and chitosan Toxicity Test using the BSLT method to see the number of deaths of Artemia salina L larvae obtained data (LC50). The results showed that the Chitosan Green Clam Shell (Perna viridis L.) had an LC50 of 4369.1806 ?g/mL. The results showed that green clam shell chitosan (Perna viridis L.) was non-toxic (LC50 > 1000 ?g/mL) in the BSLT test.

Referensi

L. Handayani and F. Syahputra, “Isolasi dan karakterisasi nanokalsium dari cangkang tiram (Crassostrea gigas),” Jphpi, vol. 20, no. 3, pp. 515–523, 2017.

N. I. Sepvina, R. Ridwanto, and Z. Rani, “Uji Toksisitas Kitosan Cangkang Kerang Bulu (Anadara antiquata) Dengan Metode Brine Shrimp Lethality Test (BSLT),” Jurnal Ilmiah Ibnu Sina, vol. 7, no. 2, pp. 380–389, 2022.

R. Ridwanto, G. I. Dalimunthe, and A. S. Daulay, “Penggunaan Kitosan Alami Hasil Isolasi Sebagai Pengawet dan Sifat Toksik Berdasarkan Uji Sitotoksisitas dengan Metode Brine Shrimp Lethality,” in Prosiding Seminar Nasional Hasil Penelitian, 2022, pp. 482–488.

N. Nurhayati, R. Ridwanto, A. S. Daulay, R. A. Syahputra, and Z. Rani, “Utilization of Chitosan As A Natural Preservative Against Catfish,” International Journal of Science, Technology & Management, vol. 3, no. 5, pp. 1396–1401, 2022.

R. Lestari, D. I. Lufita, L. Yuliatun, and L. Destiarti, Nanomaterial Dan Analisis Risiko Terhadap Lingkungan. Deepublish, 2023.

Z. Rani et al., “Cytotoxicity Test of Cocoa Leaf Ethanol Extract (Theobroma Cacao L.) With Brine Shrimp Lethality Test (BSLT) Method,” Indonesian Journal of Chemical Science and Technology (IJCST), vol. 5, no. 2, pp. 80–87, 2022.

N. Z. Arsyi, E. Nurjannah, D. Nurahlina, and E. Budiyati, “Karakterisasi nano kitosan dari cangkang kerang hijau dengan metode gelasi ionik,” Jurnal Teknologi Bahan Alam, vol. 2, no. 2, pp. 106–111, 2018.

E. J. Dompeipen, M. Kaimudin, and R. P. Dewa, “Isolasi kitin dan kitosan dari limbah kulit udang,” Majalah Biam, vol. 12, no. 1, pp. 32–39, 2016.

D. S. Saragih, R. Ridwanto, A. S. Daulay, D. Miswanda, and H. M. Nasution, “Toxicity Test of Windu Shrimp (Penaeus monodon) Skin Chitosan with Brine Shrimp Lethality Test Method,” Indonesian Journal of Chemical Science and Technology (IJCST), vol. 5, no. 2, pp. 88–93, 2022.

Z. Rani, H. M. Nasution, V. E. Kaban, N. Nasri, and N. B. Karo, “Antibacterial activity of freshwater lobster (Cherax quadricarinatus) shell chitosan gel preparation against Escherichia coli and Staphylococcus aureus,” Journal of Applied Pharmaceutical Science, vol. 13, no. 2, pp. 146–153, 2023.

S. Agustina, I. M. D. Swantara, and I. N. Suartha, “Isolasi kitin, karakterisasi, dan sintesis kitosan dari kulit udang,” Jurnal Kimia, vol. 9, no. 2, pp. 271–278, 2015.

A. Fadli, D. Drastinawati, O. Alexander, and F. Huda, “Pengaruh rasio massa kitin/naoh dan waktu reaksi terhadap karakteristik kitosan yang disintesis dari limbah industri udang kering,” Jurnal sains materi Indonesia, vol. 18, no. 2, p. 61, 2018.

R. Widyasari, D. Yuspitasari, W. Wildaniah, and R. C. Wahida, “Uji Toksisitas Akut Ekstrak Metanol Kulit Buah Jeruk Sambal (Citrus microcarpa Bunge) Terhadap Larva Artemia salina L. Dengan Metode Brine Shrimp Lethality Test (BSLT),” Medical Sains: Jurnal Ilmiah Kefarmasian, vol. 3, no. 1, pp. 51–58, 2018.

F. Fadli, S. Suhaimi, and M. Idris, “Uji Toksisitas Akut Ekstrak Etanol Daun Salam (Syzygium polyanthum (Wight) Walp.) Dengan Metode BSLT (Brine Shrimp Lethality Test),” Medical Sains: Jurnal Ilmiah Kefarmasian, vol. 4, no. 1, pp. 35–42, 2019.

L. H. Rahayu and S. Purnavita, “Optimasi pembuatan kitosan dari kitin limbah cangkang rajungan (Portunus pelagicus) untuk adsorben ion logam merkuri,” Reaktor, vol. 11, no. 1, pp. 45–49, 2007.

R. Supriningrum, S. Sapri, and V. A. Pranamala, “Uji Toksisitas Akut Ekstrak Etanol Akar KB (Coptosapelta tomentosa Valeton ex K. Heyne) Dengan Metode Brine Shrimp Lethality Test (BSLT),” Jurnal ilmiah manuntung, vol. 2, no. 2, pp. 161–165, 2016.

Unduhan

Diterbitkan

2023-10-30

Terbitan

Vol 5 No 5 (2023): J. Sains Kes.

Bagian

Articles

Cara Mengutip

Isolation and Toxicity Test of Chitosan from Green Mussels (Perna viridis L.) With Brine Shrimp Lethality Test (BSLT) Method: Study Program of Pharmacy, Faculty of Pharmacy, Universitas Muslim Nusantara Al Washliyah, Medan, Indonesia. (2023). Jurnal Sains Dan Kesehatan, 5(5), 759-765. https://jsk.ff.unmul.ac.id/index.php/JSK/article/view/588

Artikel paling banyak dibaca berdasarkan penulis yang sama