Identifikasi Gen Pengatur Sentral pada Stroke Iskemik Menggunakan Pendekatan Berbasis Jaringan

Identification of Central Regulatory Genes in Ischemic Stroke Using a Network-Based Approach

Authors

DOI:

https://doi.org/10.30872/jsk.v7i2.1053

Keywords:

Stroke iskemik, bioinformatika, gen pengatur sentral

Abstract

Stroke iskemik merupakan salah satu penyebab utama kematian dan disabilitas jangka panjang di dunia, dengan mekanisme patogenesis yang kompleks melibatkan proses inflamasi, respons imun, dan stres seluler. Penelitian ini bertujuan untuk mengidentifikasi gen pengatur sentral yang berperan dalam stroke iskemik menggunakan pendekatan bioinformatika berbasis jaringan. Data ekspresi gen diperoleh dari basis data publik dan dianalisis untuk mengidentifikasi differentially expressed genes (DEGs) dengan kriteria adjusted p-value < 0,05 dan |log2 fold change| ≥ 1. Analisis pengayaan fungsional dilakukan menggunakan Gene Ontology (GO) dan Kyoto Encyclopedia of Genes and Genomes (KEGG). Selanjutnya, jaringan protein–protein interaction (PPI) dikonstruksi dan dianalisis untuk mengidentifikasi gen hub berdasarkan topologi jaringan. Hasil analisis menunjukkan sebanyak 312 DEGs, yang didominasi oleh gen yang terlibat dalam jalur inflamasi dan imun, seperti cytokine signaling dan NF-κB signaling. Analisis jaringan mengidentifikasi gen pengatur sentral, termasuk TNF, STAT3, IL6, CXCL8, dan TYROBP, yang memiliki tingkat konektivitas tinggi. Temuan ini memberikan wawasan mengenai mekanisme molekuler stroke iskemik serta potensi biomarker dan target terapi, meskipun diperlukan validasi eksperimental lebih lanjut.

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Published

2026-05-28

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Vol. 7 No. 2 (2026): J. Sains Kes.

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How to Cite

[1]
N. Sudiyono, W. William, and Ian Pranandi, “Identifikasi Gen Pengatur Sentral pada Stroke Iskemik Menggunakan Pendekatan Berbasis Jaringan: Identification of Central Regulatory Genes in Ischemic Stroke Using a Network-Based Approach”, J. Sains. Kes, vol. 7, no. 2, pp. 309–317, May 2026, doi: 10.30872/jsk.v7i2.1053.