Uji In Vitro Ekstrak n-Heksana Rimpang Curcuma mangga Val. terhadap Ekspresi VEGF pada sel 4T1

In Vitro Test of Curcuma mangga Val. Rhizome’s n-Hexane Extract in VEGF Expression on 4T1 Cells

Authors

  • Nurul Mukhlisa Program Studi Farmasi, Fakultas Farmasi, Universitas Megarezky Makassar, Indonesia Author https://orcid.org/0009-0003-5831-0220
  • Sitti Hadijah Program Studi Farmasi, Fakultas Farmasi, Universitas Megarezky Makassar, Indonesia Author

DOI:

https://doi.org/10.30872/jsk.v5i5.593

Keywords:

TNBC, Curcuma mangga Val, VEGF, 4T1

Abstract

Kanker payudara merupakan salah satu penyakit penyebab kematian utama pada wanita di dunia. Prevalensi kanker payudara cenderung meningkat setiap tahunnya. Jumlah kasus di Indonesia mencapai 25.208 kasus dimana 20% merupakan Triple Negative Breast Cancer (TNBC). VEGF merupakan marker angiogenesis yang krusial dalam menstimulasi proliferasi sel endotel dan mengatur permeabilitas pembuluh darah yang memiliki peran penting dalam pertumbuhan, invasi, dan metastasis kanker payudara. Rimpang Curcuma.mangga Val. secara empirik digunakan masyarakat sebagai agen antikanker payudara. Ekstrak n-heksana Curcuma mangga Val. memiliki aktivitas sitotoksik terhadap sel T47D dan MCF-7. Namun, belum ada penelitian yang menunjukkan bahwa ekstrak n-heksana Curcuma mangga Val. mempunyai aktivitas sitotoksik pada sel kanker payudara TNBC. Pada penelitian ini dilakukan uji sitotoksisitas ekstrak n-heksana Curcuma mangga Val. terhadap sel 4T1 secara MTT Assay dan pengujian imunositokimia penghambatan angiogenesis ekpresi protein VEGF. Hasil pengujian sitotoksisitas menunjukkan bahwa ekstrak n-heksana Curcuma mangga Val. bersifat sitotoksik terhadap sel 4T1 dengan nilai IC50 63,37 ?M; sedangkan nilai IC50 alpelisib (kontrol sel) sebesar 32,96 ?M. Uji imunositokimia menunjukkan bahwa tidak ada perbedaan signifikan antara ekstrak n-heksana rimpang Curcuma mangga Val. dan alpelisib dalam penghambatan ekspresi VEGF. Sehingga disimpulkan bahwa ekstrak n-heksana rimpang Curcuma mangga Val. memiliki potensi antikanker TNBC melalui penghambatan protein VEGF.

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Published

2024-11-15

Issue

Vol. 5 No. 5 (2023): J. Sains Kes.

Section

Articles

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How to Cite

[1]
N. . Mukhlisa and S. . Hadijah, “Uji In Vitro Ekstrak n-Heksana Rimpang Curcuma mangga Val. terhadap Ekspresi VEGF pada sel 4T1: In Vitro Test of Curcuma mangga Val. Rhizome’s n-Hexane Extract in VEGF Expression on 4T1 Cells”, J. Sains. Kes, vol. 5, no. 5, pp. 708–715, Nov. 2024, doi: 10.30872/jsk.v5i5.593.

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