Karakterisasi Fungi Endofit Syzygium polyanthum (Wight) Walp. Berdasarkan Gen ITS Sebagai Penghasil Senyawa Antibakteri

Endophytic Fungi Characterization Syzygium polyanthum (Wight) Walp. Based on ITS Genes as Producers of Antibacterial Compounds

Authors

  • Asti Vebriyanti Asjur Universitas Megarezky, Makassar Author

Keywords:

Syzygium polyanthum, fungi endofit, antimikroba, analisis filogenetik, Penicillium sp. Colletotrichium

Abstract

Penelitian ini diawali dengan menumbuhkan dari daun salam yang telah disterilkan menggunakan etanol 70% dan natrium hipoklorit. Skrining antibakteri dilakukan dengan menggunakan uji antagonis sebagai screening pertama melawan Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Salmonella thyposa. Penelitian ini menghasilkan 2 isolat fungi. Fungi endofit aktif difermentasi dan ekstraksi dengan etil asetat (EtOAc) dan metanol. Aktivitas antibakteri dari ekstrak EtOAc dan metanol diuji aktivitasnya menggunakan metode difusi disk. Satu isolat fungi endofit (XP1) terhadap lima dari bakteri yang diuji adalah S. aureus, P. Aeruginosa, E. Coli, S. thyposa, dan B.subtilis, memiliki daya hambat terbesar pada bakteri uji S. thyposa dengan diameter daya hambat sebesar 14,00 mm. Sedangkan pada isolat fungi endofit (XP2) terhadap lima dari bakteri yang diuji adalah S. aureus, P. Aeruginosa, E. Coli, S. thyposa, dan B.subtilis, memiliki daya hambat terbesar pada bakteri uji S. thyposa dengan diameter daya hambat sebesar 22,00 mm. Berdasarkan analisis filogenetik isolat XP 1 memiliki kemiripan dengan Penicillium sp. (homology 87%) sedangkan isolat XP2 memiliki kemiripan dengan Colletotrichium gloeosporioides (homology 98%).

References

Baylis, C.L., Penn, C.W., Thielman, N.M., Guerrant, R.L., Jenkins, C., Gillespie, S.H., 2006. Eschericia coli and Shigella spp., dalam Gillespie, Hawkey, P.M. (Eds.), Principles and Practice of Clinical Bacteriology, 347-365

Burhamzah, R., G. Alam, and H. Rante. "Characterization of Antibacterial-Producing Endophytic Fungi of Syzygium polyanthum Leaves." Infectious disorders drug targets (2018).

Bolouiri, M., Sadiki, M., dan Ibnsouda, S.K. 2016. Method for in vitro evaluating antimicrobial activity : A Review. Journal of Pharmaceutical Analysis 6 (2016) : 71-79

Devi P, Rodriguez C, Naik CG, D’ Souza L. 2012. Isolation and Characterization of Antibacterial compound from a Mangrove-Endophytic Fungus, Penicilium chrysogenum MTCC 5108. Indian J. Microbiol 2012 Dec; 52 (4): 617-623

Ediningsari, Anisa R. 2008. Identifikasi khamir dari perairan mangrove dan laut cagar alam pulau rambut berdasarkan daerah Internal Transcribed Spacer (ITS).

Frohlich, J., Hyde, K. D., Petrini, O. 2000. Dalam: Guo, L.D., K.D. Hyde and E.C.Y, Liew. 2000. Identification of endophytic fungi from Livistona Chinensis based on morphology and rDNA sequences, Research New Phytol. 147 :617-630

Guo, L.D., K.D. Hyde and E.C.Y, Liew. 2000. Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequnces. Research new phytol. 147:617-630

Hillis, D.M., B.K. Marble and C. Moritz. 1996. Applications of molecular systematics: The state of the field and a look to the future. Molecular systematics.2nd Ed. Sinauer Associates Inc. Sunderland.

Jain M, Nijhawan A, Tyagi AK, Khurana JP (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochemical and Biophysical Research Communications 345: 646–651.

Kuncoro, H., & Sugijanto, N. E. (2011). Jamur Endofit, Biodiversitas, Potensi Dan Prospek Penggunaannya Sebagai Sumber Bahan Obat Baru. Journal of Tropical Pharmacy and Chemistry, 1(3), 247-262.

Lau, K.Y., Zainin, N.S., Abas, F., dan Rukayadi. Y. 2014. Antibacterial and Sporicidal Activity of Eugenia polyantha Wight. Against Bacilus cereus and Bacilus subtilis. International Journal of Current Microbiology and Applied Science Vol 3 Number 12 (2014) pp. 499-510.

Menpara, D. Dan Chanda, S. 2013. Endophytic bacteria –unexplored reservoir of antimcrobials for combating microbial pathogens Microbial pahtogens and strategies for comabating them: science, tecnology and education (A. Mendez Villaz Ed.)

Meyer A., Todt C., Mikkelsen N.T., and Lieb B., 2010, Fast evolving 18S rRNA sequences from Solenogastres (Mollusca) resist standard PCR amplification and give new insights into mollusk substitution rate heterogeneity, BMC Evolutionary Biology 10 : 10-70.

Nei, M. and S. Kumar. 2000. Molecular evolution and phylogenetics. Oxford University Press Inc. New York.

Nicot N, Hausman JF, Hoffmann L, Evers D (2005) Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. J Exp Bot 56(421): 2907–2914.

Patel, J. B. 2001. 16S rRNA gene sequencing for bacterial pathogen identification in the clinical laboratory. Mol Diagn. 6:313-321.

Prasetyoputri, A.; & Atmosukarto, I. 2006, Mikroba Endofit: Sumber Molekul Acuan Baru yang Berpotensi. BioTrends. Vol I. No.2. 2006: 13-15 2013. Identification and Bioactive Potential of Endophytic Fungi Isolated from Selected Plants of the Western Himalayas. Springer plus 2013 2:8

Radji, M. (2012). Peranan bioteknologi dan mikroba endofit dalam pengembangan obat herbal. Pharmaceutical Sciences and Research (PSR), 2(3), 113-126.

Rizki MI dan Hariandja EM. 2015. Review: Aktivitas Farmakologis, Senyawa Aktif, dan Mekanisme Kerja Daun Salam (Syzygium polyanthum) . Prosiding seminar nasional & workshop “Perkembangan Terkini Sains Farmasi dan Klinik 5” Padang 6-7 November 2015.

Schulz, B.; & Boyle, C. 2006, What Are Endophytes? dalam: Schulz, B.; Boyle, C.; & Sieber, T.N. (Eds.). Soil Biology. Volume 9. Microbial Root Endophytes. Springer-Verlag. Berlin Heidelberg. 2006: 1-13.

Singh, D., Rathod, V., Ninganagouda, S., Herimath, J., & Kulkarni, P. (2013). Biosynthesis of silver nanoparticle by endophytic fungi Pencillium sp. isolated from Curcuma longa (turmeric) and its antibacterial activity against pathogenic gram negative bacteria. Journal of pharmacy research, 7(5), 448-453.

Strobel, G.A.; Daisy, B.; Castillo, U.; & Harper, J. 2004, Natural Product from Endophytic Microorganism. J. Nat. Prod. 2004. 67: 257-268

Tamura K, Strecher G, Peterson D, Filipski A and Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013; 30: 2725-2729.

Tan, R.X.; & Zou, W.X. 2001, Endophytes: a rich source of functional metabolites. Nat. Prod. Rep.18 : 448-459

Tejesvi, M.V.; Nalini, M.S.; Mahesh, B.; Prakash, H.S.; Kini, K.R.; Shetty, H.S.; & Ven, S. 2007, New Hopes From Endhopythic Fungal Secondary metabolite. Bol. Soc. Quím. Méx., 1(1): 19-26

Travers, A., & Muskhelishvili, G. (2015). DNA structure and function. The FEBS journal, 282(12), 2279-2295.

Downloads

Published

2024-11-14

Issue

Vol. 3 No. 3 (2021): J. Sains Kes.

Section

Articles

Deprecated: json_decode(): Passing null to parameter #1 ($json) of type string is deprecated in /home/jskff/public_html/plugins/generic/citations/CitationsPlugin.php on line 68

How to Cite

[1]
A. V. Asjur, “Karakterisasi Fungi Endofit Syzygium polyanthum (Wight) Walp. Berdasarkan Gen ITS Sebagai Penghasil Senyawa Antibakteri: Endophytic Fungi Characterization Syzygium polyanthum (Wight) Walp. Based on ITS Genes as Producers of Antibacterial Compounds”, J. Sains. Kes, vol. 3, no. 3, pp. 404–409, Nov. 2024, Accessed: Feb. 18, 2026. [Online]. Available: https://jsk.ff.unmul.ac.id/index.php/JSK/article/view/223

Similar Articles

101-110 of 264

You may also start an advanced similarity search for this article.